Immunovision hct-0100
Witryna(HCT-0100, Immunovision, 1:500) and an Alexa Flour 555 goat anti-human secondary antibody (A21433, 1:500, Invitrogen, Life Technologies). Vectashield with DAPI (H-1200, Vector Laboratories) was used as a mounting medium. The cells were scanned using a Leica AF 6000 inverted fluorescence microscope, equipped with a HCX PL APO … Witrynaa human anti-centromere (1:400 for IF, HCT-0100; Immunovision, Springdale,AR,USA),andnormalmouseIgG(negativecontrolfor IP, sc-2025; Santa Cruz Biotechnology). The following secondary antibodies were used: for WB, horseradish peroxidase–conjugated donkey anti-mouse IgG (1:2000, 7076S; Cell Signaling …
Immunovision hct-0100
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WitrynaFigure Legend Snippet: A − C Asynchronous cells were stained with Hoechst, CREST, and anti-Aurora B-pT232 or anti-Aurora B antibodies. In total, 90 kinetochores from 30 … WitrynaMitotic kinetochores are initially captured by dynamic microtubules via a ‘search-and-capture’ mechanism. The microtubule motor, dynein, is critical for kinetochore capture as it has been shown to transport microtubule-attached chromosomes towards the spindle pole during early mitosis. The microtubule-binding Ndc80 complex that is recruited to …
Witryna15 cze 2024 · Chromosome instability (CIN) is a major hallmark of cancer cells and believed to drive tumor progression. Several cellular defects including weak centromeric cohesion are proposed to promote CIN, but the molecular mechanisms underlying these defects are poorly understood. In a screening for SET protein levels in various cancer … Witryna23 lip 2011 · Mouse oocyte collection. To maximize the number of antral follicles isolated from each mouse, intraperitoneally inject sexually mature female mice (we use 6 …
Witryna14 lip 2024 · Nti-histone H2A (0746, Millipore) and CREST anti-centromere serum (HCT-0100, Immunovision). Dylight series secondary antibodies (Thermo) July 14, 2024 ikk inhibitor. supernatant was collected and protein concentrations have been measured using the BCA assay (Thermo Scientific). For isolation in the cytoplasmic and … Witryna16 gru 2012 · Timely dissolution of sister-chromatid cohesion in mitosis ensures accurate chromosome segregation to guard against aneuploidy and tumorigenesis. The …
Witryna8 sty 2024 · Antibody staining was done using the following antibodies at the indicated dilutions: human CREST serum autoimmune antibody against centromere (Immunovision, HCT-0100, 1:100), mouse monoclonal anti-alpha-tubulin (DM1A) coupled to FITC (Sigma-Aldrich, F2168, 1:100), rat monoclonal anti-H3pS28 (Abcam, …
cities in georgia that start with rWitrynaAbcam ab15093 Ab15093, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, … diarrhea with hand foot and mouthWitryna16 gru 2024 · p+g 备件 sls190/0100/l/50/01/n kuka 备件 197802 asm 位移传感器 ws10-250-420a-l10-sb0-d8 emg 控制面板 ecu 01.5 multi contact 插头 tid150/2-s8/m8 30.0039 e+h 备件 cos11d-7ba21 ... (hct-b5) phoenix 总线电缆 sac-5p- 2.0-920/fs sco - 1518216 ziehl-abegg 电机 mk137-2dk.15.n hydac 滤芯 0140d010bh4hc socla 备件 cv418 … cities in georgia that start with a bWitrynaACA Immunovision HCT-0100 1:5000 Pericentrin Abcam Ab4448 1:750 Plk1 Santa Cruz SC-17783 1:1000 1:1000 Flag Sigma F1804 1:500 dsRed Clontech 632496 1:1000 Cyclin B1 BD Biosciences 554177 1:2000 pS170-Cyk4 Home brewed (Burkard et al., 2009) 1:500 Mklp1 Santa Cruz SC-867 1:350 ... diarrhea with heart failureWitrynaArticle Title: A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint Journal: Genes & Development doi: 10.1101/gad.184184.111 diarrhea with diverticulosisWitrynaAbcam mouse plasma albumin Mouse Plasma Albumin, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO … cities in germany starting with rWitryna29 paź 2016 · CREST serum (ImmunoVision, HCT-0100). 18. Fluorescent secondary antibodies . 19. 4′,6-diamidino-2-phenylindole (DAPI). 20. Plastic coverslip. Fold in the middle and use for antibody incubation. 21. Shandon Cytospin centrifuge (Thermo Scientific). 22. Shandon Double Cytoslides microscope slides (Thermo Scientific). 23. cities in germany starting with e