Fitc-conjugated pac-1
WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 … WebThe FITC-conjugated antibodies employed in a laboratory for the DFA test may be developed in house or commercially supplied. Some laboratories use a cocktail of monoclonal antibodies raised in mice against prominent antigens (e.g., nucleoprotein or glycoprotein), while others use a polyclonal antirabies antiserum raised in suitable …
Fitc-conjugated pac-1
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WebThe PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, α IIb β 3) complex of activated platelets at or near the platelet fibrinogen receptor. WebFeb 16, 2024 · The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC …
WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation. WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate.
WebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and … WebThe PAC-1 Antibody FITC has been validated for the following applications: Flow Cytometry. Flow cytometry of PAC-1 in PHA-activated human peripheral blood. Sample was incubated with PAC-1 monoclonal antibody (Product # MA5-28564) conjugated to FITC.
WebCells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated ...
WebKLH conjugated synthetic peptide derived from human AMPD3: Concentration - 100 μg (in 100 μl): 1 mg/ml: Form/Appearance: Lyophilized or Liquid: Conjugation: FITC: MW: 89kDa: UniProt ID: O08739, Q01432, O09178: Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. dln salon \\u0026 spaWebPac 1 Fitc Conjugate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more dlms ncua.govWebNov 19, 2014 · FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2 were prepared, and their integrin αvβ3/αvβ5 binding affinity was determined using the displacement assay against (125)I-echistatin bound to U87MG glioma cells. IC50 values of FITC-Galacto-RGD2, FITC-3P-RGD2, and FITC-RGD2 were calculated to be 28 ± 8, 32 ± 7, and 89 ± 17 nM, … dln nasa govWeball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ... dlmis driving licence punjabWebThe PAC-1 antibody reacts with activation-induced conformational epitope of the integrin a IIb ß 3. PAC-1 binding to fibrinogen is pH and Ca 2+ dependent, and will not bind to platelets that have been fixed. We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher. dlna dmr azamericaWebphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and fluorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA). dlna jellyfinWebProduct Specific Information. The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on … dlna platinum